Endometrial Organoids and Implantation

Successful pregnancy depends on precise communication between the embryo and endometrium, a process that can be disrupted by genetic, environmental, and drug-related factors. This project aims to deepen understanding of early embryo–endometrium interactions and the impact of toxins using advanced sheep and baboon endometrial organoids (EOs). These 3D models closely replicate the structure and function of the endometrium, enabling detailed study of embryo implantation and development under physiological and toxicological conditions. Objectives include developing robust EO generation protocols, optimizing culture conditions, and validating their similarity to in vivo tissue through morphological and molecular analyses. By examining nutrient exchange, hormonal signaling, and responses to drugs or environmental contaminants, the study seeks to reveal mechanisms that influence implantation success. Outcomes are expected to inform personalized reproductive medicine, guide safer therapeutic interventions, and provide innovative platforms for toxicology testing, ultimately advancing reproductive health and fertility outcomes.

Pediatrics and Women Center of Excellence at KFSHRC.