Molecular Diagnostics for Pathogens in Organ Transplant

This project focuses on the development of high-performance polymerase chain reaction (PCR) assays for the detection of pathogens in organ transplantation. By ensuring rigorous screening of donors and organs, we aim to mitigate infection risks for recipients. Our methodology utilizes an exhaustive pathogen panel and a thorough bioinformatic screening to design oligos with superior sensitivity and specificity. Central to this initiative is the integration of multiplexing capabilities, allowing for the simultaneous detection of multiple viral, bacterial, and fungal targets within a single reaction.

Once validated, these assays will be manufactured locally as clinical diagnostic kits, directly supporting transplant operations and enhancing patient safety through comprehensive, high-throughput screening. Expected impact of this project includes:

• Economic Efficiency: Significant cost savings by eliminating international shipping and third-party laboratory testing, and by reducing reagent consumption through multiplexed testing.

• Clinical Agility: Accelerated turnaround times and streamlined laboratory workflows, enabling rapid clinical decision-making and improved patient outcomes.

• Data Integrity: Enhanced diagnostic accuracy by reducing sample degradation and providing a comprehensive pathogen profile from a single patient sample.

• Strategic Autonomy: Strengthened national diagnostic infrastructure and reduced reliance on foreign entities through the localized production of complex, multi-target assays.